11 Mar

Serological Typing

The antigens of a microorganism can be determined by a procedure called serological typing also known as sero typing.  This involves adding a suspension of microorganism to antiserum that contains antibodies specific for antigens associated with the microorganism.  If antigens are present, the antibodies in the antiserum will react with the antigens on the bacterial cell and cause the cells to agglutinate or form visible clumps.  Serotyping comes in handy when identifying Salmonella and Shigella, that cause infections in humans such as typhoid and bacillary dysentery. Salmonella can be differentiated into more than 2500 different serotypes based on antigenic differences associated with the cell.  Serotyping of Salmonella and Shigella is useful in tracing epidemics caused by a particular strain of serotype of the respective organism. pauln