18 Mar

Slide Agglutination Test

The slide agglutination test determines whether or not a specific protein exists within a species.  Many manufacturers of reagents for slide agglutination tests utilize polystyrene latex particles as carriers for the antibody molecules.  By conjugating reactive antibodies to these particles, an agglutination reaction results that occurs rapidly and is much easier to see than ordinary precipitin-type reactions that are used to demonstrate the presence of a soluble antigen.  The test reagent is a suspension of yellow latex particles sensitized with antibodies for coagulase and protein A.  Reagents are used to provide negative and positive controls.  Disposable cards with eight black circles printed on them for performing the test.  The black background of the card provides good contrast for the agglutination reaction that occurs. pauln