Monthly Archives: May 2011

31 May

UPLC

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Ultra Performance Liquid Chromatography is related to HPLC (High Performance or High Pressure Liquid Chromatography) but uses smaller particle sizes (less than 2 μm.) and smaller diameter columns.  The efficiency of HPLC separation is inversely related to particle size.  Unfortunately the smaller … Continue reading

27 May

Aseptic Technique

Aseptic technique ensures that no contaminating organisms are introduced into culture materials when these materials are innoculated.  It also prevents the handler of the organisms from being contaminated.  Aseptic technique can be used to transfer broth culture to a plate … Continue reading

23 May

What is Cold?

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We say our freezer temperature is cold.  The ultra low freezers are even colder.  Standard laboratory freezers reach temperatures of – 20°C.  There are two ranges for ultra-low Freezers; -40 to -10°C and -86 to -40°C.  Colder temperatures can be reached in the … Continue reading

20 May

Fungi

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Fungi are heterotrophic eukaryotic organisms that produce exoenzymes and absorb their nutrients.  Fungi may be saprophytic or parasitic and can be unicellular or filamentous.  The features that separate fungi from other organisms are: eukaryotic, heterotrophic, lack tissue differentiation, have cell … Continue reading

16 May

Spectroscopy

Spectroscopy is the study of how radiated energy interacts with matter.  Spectroscopy started with UV & Vis spectroscopy (Ultraviolet and visible light), then infrared; now most parts of the electromagnetic spectrum are passed through matter to study the interactions.  Atoms and molecules have … Continue reading

13 May

Shape and Structure of Bacteria

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The shape and structure of bacteria make it easy to identify them.  The structure of bacteria is simple.  They have supercoiled DNA in the cytoplasm and are able to carry out photosynthesis and respiration.  Their cell wall is made of … Continue reading

09 May

Balance/Scale

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A lab balance is used to precisely measure reagents, standards, and samples.  The word scale generally refers to instruments for weighing higher masses than a balance.  A balance uses an arm or beam to balance between known calibrated weights and … Continue reading

06 May

Infectious Disease

A disease casued by microorganisms (such as bacteria, viruses) that enter the body and multiply in the tissues at the expesne of the host is said to be an infectious disease.  Infectious diseases that are transmissible to other people are called … Continue reading