27 Jul

Warthin-Starry Stain

Warthin – Starry Stain, developed by: Warthin, Aldred Scott (1866–1931), and Starry, Allen Chronister (born 1890), American pathologists.   Developed for the diagnosis of bacteria such as: Microsporidial infectionsSpirochetes and Cat Scratch Fever, which prior to reading about this study, I always thought was just a song written by 70′s Rockstar, Ted Nugent. 

The Warthin-Starry Stain, which introduced silver nitrate as a method for demonstrating spirochetes in tissues proved to be a reliable and sensitive diagnostic technique.

  • Microsporidiosis, which is small intestinal bacteria in HIV-infected patients.
  • Spirochetes are a group of phylogenetically-distinct bacteria which are widespread in viscous environments and found in intestinal tracts of animals and oral cavity of humans
  • Cat Scratch Fever, also known as (Bartonella) is characterized by chills, fever and swelling of the lymph glands.  As stated by its name, is transmitted by a cat scratch.  Cats transmit the organism when they are parasitized by fleas, scratch themselves, and get infected flea dirt in their claws, and scratch a person or another cat with their dirty claws.

The above picture is an example of the technique for the demonstration of Bacillus Piliformis. Piliformis stains black and the background is light brown to golden brown.  Depending on the numbers of tests run, each kit will provide an average of 100 – 200 test slides.