31 Oct

Columns for HPLC Separation of Peptides

Reverse phase HPLC does not always provide satisfactory separation of closely related peptides and proteins.  The Sielc Promex series of chromatography columns are specifically designed for the separation of peptides and proteins.  The stationary phase combines an ionic (cation or anion) group with a hydrophobic chain.  This specialized stationary phase is 2D chromatography, (reverse phase and ionic) in one column.  Promix columns have the ability to separate peptides and proteins differing in only one amino acid.   The enhanced selectivity allows for separation of closely related peptides and proteins.   Promix columns can be used with formic acid and ammonium acetate mobile phase for high LC-MS sensitivity.  Sielc Promix series columns are available from capillary to preparative.