07 May

Homogenization

Sample preparation often depends on a homogenizer or disperser to suspend the sample in preparation for analysis.   The choice of homogenizer will depend on the volume of sample and the nature of the sample.  Rotor/stator type dispersers can handle volumes from 50µl up to 40L.  The rotor/stator homogenizer is ideal for tough fibrous tissue and brittle pharmaceutical samples.  The rotor/stator homogenizer has a powerful motor which can operate from 6000 to 24,000 rpm.  The size of the motor needed will depend on the volume of sample; power can range from 100 watts to 1700 watts or more.

The working portion of the homogenizer is the rotor/stator, also called a dispersing element or generator.  The inside of this element turns (rotor) and the outside is stationary (stator).  The rotor/stator can be designed for tough fibrous materials such as plant and animal tissue, or brittle materials such as pharmaceutical tablets.  In some cases it may be necessary to start with one style element and change to another for the most efficient dispersion of the sample.  Manufacturers such as Kinematica and IKA Works have support which will help the user choose the best homogenizer motor and rotor/stator for their specific application.

Robin Prymula