13 Jul

Blood Typing

Blood typing may be performed by slide or tube methods.  In my previous post I discussed the different blood groups and how they are differentiated.  The procedure for blood typing was devloped in 1900 by Karl Landsteinter.  He is credited with having discovered that blood types can be separated into 4 groups.  Blood typing is performed with antisera containing high levels of anti-A and anti-B antibodies.  In both cases a drop of each kind of antiserum is added to separate samples of saline suspension of red blood cells.  If agglutination occurs in the suspension to which anti-a serum was added, the blood is type A.  If the agglutination occurs in the suspension to which the anti-b serum was added, the blood type is B.  If both suspension experience agglutination, the blood type is AB.  The absence of agglutination means type O blood.  One may put the sample of blood on a microscope slide and add the different types of serum.